Effects of Neutral, Cationic, and Anionic Chromium Ascorbate Complexes on Isolated Human Mitochondrial and Genomic DNA

The relative activities of neutral, cationic, and anionic chromium ascorbate complexes toward isolated human mitochondrial and genomic DNA were investigated at physiologically relevant conditions by agarose gel electrophoresis. A direct relationship between the charge of the Cr(III) species and thei...

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Bibliographic Details
Published inBMB reports Vol. 36; no. 4; pp. 403 - 408
Main Authors Ahmet Nedim Ay, Birgul Zumreoglu Karan, Reyhan Oner, Canan Unaleroglu, Cihan Oner
Format Journal Article
LanguageKorean
Published 생화학분자생물학회 31.07.2003
Subjects
Ascorbate
Chromium
DNA-cleavage
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Summary:The relative activities of neutral, cationic, and anionic chromium ascorbate complexes toward isolated human mitochondrial and genomic DNA were investigated at physiologically relevant conditions by agarose gel electrophoresis. A direct relationship between the charge of the Cr(III) species and their DNA-damaging properties was found. The cationic species were found to be fully capable of DNA-cleavage, even in short incubation periods. Incubations were also performed in the presence of amino acids. No apparent effect was observed under the applied experimental conditions to facilitate or prevent damage through the ternary amino acid-Cr-DNA adduct formation or binary chromium-amino acid complex formation.
Bibliography:Korean Society for Biochemistry and Molecular Biology
ISSN:1976-6696