Endothelin - 1 에 의한 돼지 관상동맥 수축에 미치는 17β - Estradiol 의 효과

• Published in: The Korean journal of medicine Vol. 52; no. 2; pp. 224 - 232
• Main Authors: 정호경, Ho Gyeong Jeong, 강병선, Byeong Sun Kang, 김민구, Min Gu Kim, 박병건, Byeong Gun Park, 황진용, Jin Yong Hwang, 서봉관
• Format: Journal Article
• Language: Korean
• Published: 대한내과학회 01.02.1997
• Subjects: 17β-estradiol; Endothelin; Porcine coronary artery
• ISSN: 1738-9364

Objectives: It is widely accepted that estrogen has favorable effects on cardiovascular diseases, especially in the postmenopausal women. Endothelin- 1(ET-I), released from the vascular endothelium, is a 21-amino acid peptide with strong vasoconstrictor activity. However, the effect of estrogen on the vasoconstriction to ET-1 has not been extensively studied. Methods: To investigate the effect of estrogen (175β-estradiol) on the vascular contraction to ET-1, porcine coronary artery(PCA) rings were suspended in organ chambers(37℃, 95% O2/5% CO2) for measurement of isometric tension change. Endothelium was removed mechanically if necessary. In acute experiments, vascular rings were preincubated for 15minutes with 3different concentrations of 170β-estradiol(10(-6), 10(-5), 10(-4)M) and concentration-contraction curves to cumulative doses of ET-1 were constructed. In the experiments after a longer exposure to 17β-estradiol, the vessels with endothelium were exposed in the 5% CO2 incubator to 3different concentrations of 17β-estradiol(10(-9), 10(-8), 10(-7) M) for 44-50 hours, and then concentrationcontraction curves to ET-1 were obtained. Results: Incubation for 15minutes with 170β-estradiol(10 -4)M) inhibited ET-1-induced contraction in the vessels with endothelium(area under the curve and maximal contraction, p<0.05 compared with control). This effect persisted regardless of the sex and the presence or absence of the endotheliurn. Incubation of the vessels far a longer time with 170β-estradiol(44-50 hours) resulted in the inhibition of maximal contraction to ET-1(p<0.05) by a lower concentration of 175β-estradiol(10(-7)M) than in acute experiments in male PCA rings, but an enhanced contraction to ET-1(area under the curve; p<0.05) by 10M of 175β-estradiol was observed in female PCA rings. Conclusion: Short-time incubation with 17Pβ-estradiol has an inhibitory effect on the contraction to ET-1 in PCA rings. This effect is independent of the presence of the endothelium and the sex of the pigs. A longer incubation with 17β-estradiol results in a similar inhibitory effect on male(but not female) PCA rings, suggesting that a sex-related difference may exist concerning the effect of 17β-estradiol on ET-1-induced contraction.