만성 B형간염에서 간 조직내 TGF-p1과 a-SMA 단백질의 발현정도와 인터페론 치료효과와의 상관관계

• Published in: Clinical and molecular hepatology Vol. 4; no. 4; pp. 317 - 329
• Main Authors: 김배영, Bai Young Kim, 안희정, hee Jeong Ahn, 오성욱, Seong Wook Oh, 정은미, Oun Mi Jeong, 최유정, Yu Jeong Choi, 국진환, Jin Hwan Kook, 김경철, Kyung Chul Kim, 홍성표, Sung Pyo Hong, 황성규, Seong Gyu Hwang, 박필원, Pil Won Park, 임규성, Kyu Sung Rim
• Format: Journal Article
• Language: Korean
• Published: 대한간학회 01.01.1998
• Subjects: A-Interferon; a-SMA; Chronic hepatitis; Predicting Indicator; TGF-p1
• ISSN: 2287-2728; 2287-285X

Backg round/Aims : The aim of this study was to evaluate the effect of α- interferon (IFN) on liver histology as well as on activation of hepatic stellate cell ( HSC) and trans for ming growth fact or β- 1 (TGF β- 1) expression. We had also investigated the clinical usefulness of TGFβ- 1 and α- smooth muscle actin (α- SMA) expression in liver tissue for predicting a response toα- IFN therapy in chronic hepat it is B. Methods : We studied the expression of TGFβ-1 and α- SMA in liver biopsys pecimens from 51 chronic hepatitis B pat ients . Using immunohistochemical staining and a semiquant it ative scoring met hod, we also evaluated TGF- β1 and α- SMA expression in liver stellate cells before and after α- IFN therapy in liver tissue from rebiopsys pecimen of the 12 chronic hepatitis B pat ients . Recombinant IFN α- 2b (Intron A) in doses of 6 MU/ d was given to patients intramus cularly three times per week for 6 months ( total doses , 432 MU). The patients were divided into two groups according to serum alanine aminotransferase levels as well as HBV- DNA and HBeAg s eroconversion stat e. Histological grading and staging scores were according to modified Histological Activity Index (HAI) gradingsys tems of Ishak ( 1995). Results : The index of portal inflammation and total scores of HAI grading significantly decreased in biopsies afterα - IFN t reat ment, but the scores of fibrosis staging showed no significant change in biopsies after IFN t reat ment. A significant decrease in α- SMA expression, especially in periportal area, was found, but the change of TGFβ- 1 expression was not significant. The immunoreactivity of α- S MA was significantly lower in responders than in non-res ponders , whereas the diffference of immun or eactivity of TGFβ - 1 between these two groups was not found. Conclusions : These findings suggest that α- IFN therapy may reduce the necroinflammatory activity in liver tissues of chronic B viral hepatitis and that the degree of - S MA ex pression before treatment may be employed as a pottent predicting indicat or for the therapeutic efficacy of IFN- alfa.(Korean J Hepatol 1998;4:317 329)