Vibrio vulnificus 의 Cell Wall Protein A 와 Lipopolysaccharide ( LPS ) 의 추출 및 이들이 효소활성에 미치는 영향

• Published in: BMB reports Vol. 27; no. 5; pp. 413 - 417
• Main Authors: 김용호, 이봉헌, 박장수, 강신원, Yong Ho Kim, Bong Hun Lee, Jang Su Park, Shin Won Kang
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 01.01.1994
• ISSN: 1976-6696

Lipopolysaccharide (LPS) and cell wall protein A from Vibrio vulnificus were extracted, were tested the lethal toxicity and Limulus amebocyte gelation activity, and were examined the effect of cell wall protein A on the enzyme activity of LPS. These results were compared to those of LPSs and cell wall protein As from Escherichia coli and Salmonella typhimurium. The lethal toxicity of V vulnificus LPS (LDSO was 138.6 ㎎/㎏) was lower than those of E. coli LPS (56.3 ㎎/㎏) and S. typhimurium LPS (37.5 ㎎/㎏), but the Limulus gelation activities of all LPSs were the same value (0.1 ng/㎖). The results of enzyme activity by LPS and cell wall protein A have shown that the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were increased. The cell wall protein A on the enzyme activities of LPS was effective; the enzyme activities of LPS-cell wall protein A mixture were greater than those of LPS only.