고려인삼의 간독성에 대한 보호성분 : 과산화지질 형성에 미치는 영향

• Published in: BMB reports Vol. 22; no. 1; pp. 12 - 18
• Main Authors: 김헤영, 이유희, 김신일, Hye Young Kim, You Hui Lee, Shin Il Kim
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 01.01.1989
• ISSN: 1976-6696

Three polyacetylene compounds, panaxydol, panaxynol and panaxytriol were isolated from Korean ginseng and their inhibitory effects on lipid peroxidation in vivo and in vitro were investigated. Lipid peroxide levels both in serum and liver microsome and serum enzyme (GOT, GPT, LDH) activities of CCl₄ -administered rats were determined after pretreatment of polyacetylenes. Hepatic microsomal cytochrome P-450-dependent monooxygenases were also measured to investigate the mechanism of action. Enzymatic (paraquat/NADPH) and nonenzymatic (ascorbate/Fe^(+2) systems were used for in vitro lipid peroxidation. As results, polyacetylene compounds inhibited liver lipid peroxidation and panaxynol lowered serum lipid peroxide levels caused by CC1₄. Polyacetylene pretreatment prevented leakage of LDH to serum but elevated GOT and GPT levels were not changed. CCl₄ caused marked losses in cytochrome P-450 and reduced the activities of aniline hydroxylase and aminopyrine demethylase. Polyacetylene treatment without CCl₄ induced aminopyrine demethylase and panaxydol and panaxynol induced aniline hydroxylase while the content of cytochrome P-450 were not affected. In vitro microsomal lipid peroxidation was inhibited by polyacetylenes and DL-α-tocopherol in a dose-dependent manner. The results demonstrate that polyacetylenes protected against hepatotoxicity of CCl₄ by inhibiting lipid peroxidation in vivo and in vitro possibly through the mechanism of direct action on liver microsomes.