재조합 인체 인터루킨 - II . 생물학적 및 면역요법적 활성도

Recombinant human interleukin-2 (rH IL-2, Ser^(125)-rH IL-2) purified from E. coli was characterized its biological and therapeutic activities for the establishment of preclinical screening system and therapeutic applications. In vitro augmentation of natural killer (NK) cell activity and mixed lymp...

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Published inBMB reports Vol. 21; no. 2; pp. 127 - 133
Main Authors 최혜림, 윤혜영, James E . Talmadge, 함경수, Hye Lim Choi, Hye Young Yun, Kyung Soo Hahm
Format Journal Article
LanguageKorean
Published 생화학분자생물학회 01.01.1988
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Summary:Recombinant human interleukin-2 (rH IL-2, Ser^(125)-rH IL-2) purified from E. coli was characterized its biological and therapeutic activities for the establishment of preclinical screening system and therapeutic applications. In vitro augmentation of natural killer (NK) cell activity and mixed lymphocyte reaction (MLR) assay were shown to be comparable with other rH IL-2 from different manufacturers and laboratories. An antiserum against rH IL-2 was obtained from chick, and characterized the reacticvity with rH IL-2 reconstituted from lyophilized state in relation to the stability and conformational changes of the recombinant protein. In addition, long term storage of rH IL-2 in lyophilized or solution was not shown to incur remarkable loss of MLR activities, and any appearance of either degradation or aggregation products when analyzed by SDS-PAGE, indicating the stability and suitability for therapeutic applications of rH IL-2.
Bibliography:Korean Society for Biochemistry and Molecular Biology
ISSN:1976-6696