Proceedinsg of 12th Scientific Meeting ; Effect of Cold Exposure on Phosphoenolpyruvate Carboxykinase of Chicken Liver

• Published in: BMB reports Vol. 10; no. 4; pp. 298 - 299
• Main Author: Joon Seung Jo
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 01.01.1977
• ISSN: 1976-6696

This study is to observe the change of phosphoenolpyruvate carboxykinase (EC 4.1.1. 32) activity of chicken liver when it was treated with cold exposure. The enzyme activity was determined by means of oxaloacetate-H^(14)CO^-₃ exchange reaction, and the various forms of the enzyme were analysed by DEAE-Sephadex chromatography. The results were summerized as follows: The enzyme was found to exist in cytosolic or mitochondrial fraction of the liver, and enzyme of each fraction of young chicken liver has been separated into two isozymic forms by means of DEAE-Sephadex column chromatography. These forms were designated as forms I and II in their order of elution from the column. In adult chicken, however, only form I of the enzyme has been found, with form II being extremely too low to be recognized as a peak. Being placed in the cold room for 21 or 18 hours, the enzyme of chicken was induced relatively low, whereas the chromatographic profile of the enzyme changed markedly, with form I reduced to low level and form II emerging predominantly. These findings suggest that isozymic forms of the enzyme were regulated independently by cold exposure.