Induction of a Sensitive Response to Helminthosporium maydis Race T Toxin in Resistant Mithochondria of Corn (Zea mays L.) by Removal of the Outer Mitochondrial Membrane

Mitochondria isolated from Texas cytoplasmically male sterile (Tms) and normal (N) versions of corn (Zea mays L.) exhibit differential sensitivity to toxin(s) produced by Helminthosporium maydis race T, the causal organism of southern corn leaf blight. Malate dehydrogenase was inhibited by toxin(s)...

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Published inPlant physiology (Bethesda) Vol. 56; no. 2; pp. 216 - 221
Main Authors Watrud, Lidia S., Joan K. Baldwin, Miller, Raymond J., Koeppe, David E.
Format Journal Article
LanguageEnglish
Published American Society of Plant Physiologists 01.08.1975
Subjects
Cell membranes
Chemical suspensions
Corn
Gels
Helminthosporium
Implementation maturity model
Liquids
Mitochondria
Mitochondrial membranes
Toxins
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Summary:Mitochondria isolated from Texas cytoplasmically male sterile (Tms) and normal (N) versions of corn (Zea mays L.) exhibit differential sensitivity to toxin(s) produced by Helminthosporium maydis race T, the causal organism of southern corn leaf blight. Malate dehydrogenase was inhibited by toxin(s) in intact Tms mitochondria but was unaffected in N mitochondria. Removal or rupture of the outer mitochondrial membrane resulted in retention of sensitivity of malate dehydrogenase in Tms mitochondria to toxin(s), and induction of a sensitive response in normally toxin-insensitive N mitochondria. This suggests that a permeability difference in the respective outer membranes of N and Tms mitochondria may affect the passage of toxin(s) to a mitochondrial site of action. Mitochondrial bioassays indicate that more toxin was bound by Tms mitochondria than by N mitochondria; the greatest toxin binding was associated with the inner membrane of Tms mitochondria.
ISSN:0032-0889
1532-2548