A Burkholderia pseudomallei Toxin Inhibits Helicase Activity of Translation Factor elF4A

• Published in: Science (American Association for the Advancement of Science) Vol. 334; no. 6057; pp. 821 - 824
• Main Authors: Cruz-Migoni, Abimael, Hautbergue, Guillaume M., Artymiuk, Peter J., Baker, Patrick J., Bokori-Brown, Monika, Chang, Chung-Te, Dickman, Mark J., Essex-Lopresti, Angela, Harding, Sarah V., Mahadi, Nor Muhammad, Marshall, Laura E., Mobbs, George W., Mohamed, Rahmah, Nathan, Sheila, Ngugi, Sarah A., Ong, Catherine, Ooi, Wen Fong, Partridge, Lynda J., Phillips, Helen L., Raih, M. Firdaus, Ruzheinikov, Sergei, Sarkar-Tyson, Mitali, Sedelnikova, Svetlana E., Smither, Sophie J., Tan, Patrick, Titball, Richard W., Wilson, Stuart A., Rice, David W.
• Format: Journal Article
• Language: English
• Published: American Association for the Advancement of Science 11.11.2011
• Subjects: 3T3 cells; Biomedical technology; Burkholderia; Burkholderia pseudomallei; Cell extracts; Immunoblotting; Polyribosomes; RNA; Toxicity; Toxins

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Eschenchia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of...