A Burkholderia pseudomallei Toxin Inhibits Helicase Activity of Translation Factor elF4A

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Eschenchia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of...

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Published inScience (American Association for the Advancement of Science) Vol. 334; no. 6057; pp. 821 - 824
Main Authors Cruz-Migoni, Abimael, Hautbergue, Guillaume M., Artymiuk, Peter J., Baker, Patrick J., Bokori-Brown, Monika, Chang, Chung-Te, Dickman, Mark J., Essex-Lopresti, Angela, Harding, Sarah V., Mahadi, Nor Muhammad, Marshall, Laura E., Mobbs, George W., Mohamed, Rahmah, Nathan, Sheila, Ngugi, Sarah A., Ong, Catherine, Ooi, Wen Fong, Partridge, Lynda J., Phillips, Helen L., Raih, M. Firdaus, Ruzheinikov, Sergei, Sarkar-Tyson, Mitali, Sedelnikova, Svetlana E., Smither, Sophie J., Tan, Patrick, Titball, Richard W., Wilson, Stuart A., Rice, David W.
Format Journal Article
LanguageEnglish
Published American Association for the Advancement of Science 11.11.2011
Subjects
3T3 cells
Biomedical technology
Burkholderia
Burkholderia pseudomallei
Cell extracts
Immunoblotting
Polyribosomes
RNA
Toxicity
Toxins
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Summary:The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Eschenchia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor elF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.
ISSN:0036-8075
1095-9203