Transcriptional Termination by RNA Polymerase I Requires the Small Subunit Rpa 12p

We identify Rpa12p of RNA polymerase I (Pol I) as a termination factor. Combined analyses using transcription run-on, electron microscopy-visualized chromatin spreading and RT-PCR have been applied to the rRNA-encoding genes of Saccharomyces cerevisiae. These confirm that Pol I termination occurs cl...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 101; no. 16; pp. 6068 - 6073
Main Authors Prescott, Elizabeth M., Osheim, Yvonne N., Jones, Hannah S., Alen, Claudia M., Roan, Judith G., Reeder, Ronald H., Beyer, Ann L., Proudfoot, Nick J., Guthrie, Christine
Format Journal Article
LanguageEnglish
Published National Academy of Sciences 20.04.2004
Subjects
Biological Sciences
DNA
DNA probes
Genes
Nucleotides
Reverse transcriptase polymerase chain reaction
Ribosomal DNA
RNA
Signal detection
Terminator regions
Yeasts
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Summary:We identify Rpa12p of RNA polymerase I (Pol I) as a termination factor. Combined analyses using transcription run-on, electron microscopy-visualized chromatin spreading and RT-PCR have been applied to the rRNA-encoding genes of Saccharomyces cerevisiae. These confirm that Pol I termination occurs close to the Reb1p-dependent terminator in wild-type strains. However, deletion mutants for the 3′ end-processing enzyme Rnt1p or the Rpa12p subunit of Pol I both show Pol I transcription in the spacer. For Δrpa12, these spacer polymerases are devoid of nascent transcripts, suggesting they are immediately degraded. The homology of Rpa12p to the small subunit Rpb9p of Pol II and Rpc11p of Pol III, both implicated in transcriptional termination, points to a common termination mechanism for all three classes of RNA polymerase.
ISSN:0027-8424
1091-6490