Genome ofRhodnius prolixus,an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 48; pp. 14936 - 14941
• Main Authors: Mesquita, Rafael D., Vionette-Amaral, Raquel J., Lowenberger, Carl, Rivera-Pomar, Rolando, Minx, Patrick, Spieth, John, Carvalho, A. Bernardo, Panzera, Francisco, Lawson, Daniel, Torres, André Q., Ribeiro, Jose M. C., Sorgine, Marcos H. F., Montague, Michael J., Abad-Franch, Fernando, Alves-Bezerra, Michele, Araujo, Helena M., Araujo, Ricardo N., Aravind, L., Atella, Georgia C., Azambuja, Patricia, Bittencourt-Cunha, Paula R., Braz, Gloria R. C., Calderón-Fernández, Gustavo, Carareto, Claudia M. A., Christensen, Mikkel B., Costa, Igor R., Dansa, Marilvia, Daumas-Filho, Carlos R. O., De-Paula, Iron F., Dimopoulos, George, Emrich, Scott J., Esponda-Behrens, Natalia, Fampa, Patricia, Fernandez-Medina, Rita D., da Fonseca, Rodrigo N., Fontenele, Marcio, Fronick, Catrina, Gandara, Ana Caroline, Garcia, Eloi S., Giraldo-Calderón, Gloria I., Gomes, Bruno, Gondim, Katia C., Guarneri, Alessandra A., Guigó, Roderic, Harry, Myriam, Jablonka, Willy, Jacquin-Joly, Emmanuelle, Juárez, M. Patricia, Lange, Angela B., Latorre-Estivalis, José Manuel, Lavore, Andrés, Lawrence, Gena G., Lazoski, Cristiano, Lopes, Raphael R., Lorenzo, Marcelo G., Lugon, Magda D., Majerowicz, David, Marcet, Paula L., Mariotti, Marco, Masuda, Hatisaburo, Megy, Karine, Melo, Ana C. A., Missirlis, Fanis, Noriega, Fernando G., Nouzova, Marcela, Nunes, Rodrigo D., Oliveira, Raquel L. L., Ons, Sheila, Orchard, Ian, Pagola, Lucia, Paiva-Silva, Gabriela O., Pascual, Agustina, Pavan, Marcio G., Pedrini, Nicolás, Peixoto, Alexandre A., Pike, Andrew, Polycarpo, Carla, Prosdocimi, Francisco, Ribeiro-Rodrigues, Rodrigo, Robertson, Hugh M., Salmon, Didier, Santesmasses, Didac, Schama, Renata, Seabra-Junior, Eloy S., Silva-Cardoso, Livia, Silva-Neto, Mario A. C., Souza-Gomes, Matheus, Taracena, Mabel L., Tojo, Marta, Tu, Zhijian Jake, Tubio, Jose M. C., Ursic-Bedoya, Raul, Venancio, Thiago M., Walter-Nuno, Ana Beatriz, Wilson, Derek, Warren, Wesley C., Wilson, Richard K., Huebner, Erwin, Dotson, Ellen M., Oliveira, Pedro L.
• Format: Journal Article
• Language: English
• Published: National Academy of Sciences 01.12.2015
• ISSN: 0027-8424; 1091-6490

Rhodnius prolixusnot only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome ofR. prolixus,generated assembled sequences covering 95% of the genome (∼702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immunedeficiency (IMD)-mediated immune responses were observed,R. prolixusputatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affectedTrypanosoma cruzi,the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms.R. prolixushas experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes fromWolbachiasp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences inR. prolixus,including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.