Cytochromecbb₃ ofThioalkalivibriois a Na⁺-pumping cytochrome oxidase

Cytochromecoxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb₃ cytochromes), which ar...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 112; no. 25; pp. 7695 - 7700
Main Authors Muntyan, Maria S., Cherepanov, Dmitry A., Malinen, Anssi M., Bloch, Dmitry A., Sorokin, Dimitry Y., Severina, Inna I., Ivashina, Tatiana V., Lahti, Reijo, Muyzer, Gerard, Skulachev, Vladimir P.
Format Journal Article
LanguageEnglish
Published National Academy of Sciences 23.06.2015
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Summary:Cytochromecoxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb₃ cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), acbb₃ cytochrome from the extremely alkaliphilic bacteriumThioalkalivibrio versutus. This finding offers clues to the previously unknown structure of the ion-pumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme.
ISSN:0027-8424
1091-6490