Cytochromecbb₃ ofThioalkalivibriois a Na⁺-pumping cytochrome oxidase
Cytochromecoxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb₃ cytochromes), which ar...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 25; pp. 7695 - 7700 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
National Academy of Sciences
23.06.2015
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Online Access | Get full text |
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Summary: | Cytochromecoxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb₃ cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), acbb₃ cytochrome from the extremely alkaliphilic bacteriumThioalkalivibrio versutus. This finding offers clues to the previously unknown structure of the ion-pumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme. |
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ISSN: | 0027-8424 1091-6490 |