A phosphoenol pyruvate phosphatase transcript is induced in the root nodule cortex ofPhaseolus vulgarisunder conditions of phosphorus deficiency

Although previous studies on N₂-fixing legumes have demonstrated the contribution of acid phosphatases to their phosphorus (P) use efficiency under P-deficient growth conditions, localization of these enzymes in bean nodules has not been demonstrated. In this study, phosphoenol pyruvate phosphatase...

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Bibliographic Details
Published inJournal of experimental botany Vol. 63; no. 13; pp. 4723 - 4730
Main Authors Bargaz, A., Ghoulam, C., Amenc, L., Lazali, M., Faghire, M., Abadie, J., Drevon, J.J.
Format Journal Article
LanguageEnglish
Published OXFORD UNIVERSITY PRESS 01.01.2012
Subjects
Acid soils
DNA
Genes
Legumes
Nodules
Phosphatases
Phosphorus
Plants
RESEARCH PAPER
RNA
Symbiosis
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Summary:Although previous studies on N₂-fixing legumes have demonstrated the contribution of acid phosphatases to their phosphorus (P) use efficiency under P-deficient growth conditions, localization of these enzymes in bean nodules has not been demonstrated. In this study, phosphoenol pyruvate phosphatase (PEPase) gene transcripts were localized within the nodule tissues of two recombinant inbred lines, RIL115 (P-deficiency tolerant) and RIL147 (P-deficiency sensitive), ofPhaseolus vulgaris. Nodules were induced byRhizobium tropiciCIAT899 under hydroaeroponic conditions with a sufficient versus a deficient P supply. The results indicated that PEPase transcripts were particularly abundant in the nodule infected zone and cortex of both RILs. Analysis of fluorescence intensity indicated that nodule PEPase was induced under conditions of P deficiency to a significantly higher extent in RIL147 than in RIL115, and more in the inner cortex (91%) than in the outer cortex (71%) or the infected zone (79%). In addition, a significant increase (39%) in PEPase enzyme activity in the P-deficient RIL147 correlated with an increase (58%) in the efficiency of use in rhizobial symbiosis. It was concluded that nodule PEPase is upregulated under conditions of P deficiency in the P-deficiency-sensitive RIL147, and that this gene may contribute to adaptation of rhizobial symbiosis to low-P environments.
ISSN:0022-0957
1460-2431