Arabidopsis thalianaDOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14

Seed dormancy prevents seeds from germinating under environmental conditions unfavourable for plant growth and development and constitutes an evolutionary advantage. Dry storage, also known as after-ripening, gradually decreases seed dormancy by mechanisms not well understood. AnArabidopsis thaliana...

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Published inJournal of experimental botany Vol. 63; no. 5; pp. 1937 - 1949
Main Authors Rueda-Romero, Paloma, Barrero-Sicilia, Cristina, Gómez-Cadenas, Aurelio, Carbonero, Pilar, Oñate-Sánchez, Luis
Format Journal Article
LanguageEnglish
Published Oxford University Press 01.01.2012
Subjects
After ripening
Dormancy
Genes
Germination
Imbibition
Messenger RNA
Phenotypes
Plants
RESEARCH PAPER
Seed germination
Seeds
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Summary:Seed dormancy prevents seeds from germinating under environmental conditions unfavourable for plant growth and development and constitutes an evolutionary advantage. Dry storage, also known as after-ripening, gradually decreases seed dormancy by mechanisms not well understood. AnArabidopsis thalianaDOF transcription factor gene (DOF6) affecting seed germination has been characterized. The transcript levels of this gene accumulate in dry seeds and decay gradually during after-ripening and also upon seed imbibition. While constitutive over-expression ofDOF6produced aberrant growth and sterility in the plant, its over-expression induced upon seed imbibition triggered delayed germination, abscisic acid (ABA)-hypersensitive phenotypes and increased expression of the ABA biosynthetic geneABA1and ABA-related stress genes. Wild-type germination and gene expression were gradually restored during seed after-ripening, despite ofDOF6-induced over-expression. DOF6 was found to interact in a yeast two-hybrid system andin plantawith TCP14, a previously described positive regulator of seed germination. The expression ofABA1and ABA-related stress genes was also enhanced intcp14knock-out mutants. Taken together, these results indicate that DOF6 negatively affects seed germination and opposes TCP14 function in the regulation of a specific set of ABA-related genes.
ISSN:0022-0957
1460-2431