Function of von Willebrand Factor After Crossed Bone Marrow Transplanation Between Normal and Von Willebrand Disease Pigs: Effect on Arterial Thrombosis in Chimeras

von Willebrand factor (vWF) is essential for the induction of occlusive thrombosis in stenosed and injured pig arteries and for normal hemostasis. To separate the relative contribution of plasma and platelet vWF to arterial thrombosis, we produced chimeric normal and von Willebrand disease pigs by c...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 92; no. 7; pp. 2455 - 2459
Main Authors Nichols, Timothy C., Samama, C. Marc, Bellinger, Dwight A., Roussi, Jacqueline, Reddick, Robert L., Bonneau, Michel, Read, Marjorie S., Bailliart, O., Koch, Gary G., Vaiman, Marcel, Sigman, Jeff L., Pignaud, George A., Brinkhous, Kenneth M., Griggs, Thomas R., Drouet, Ludovic
Format Journal Article
LanguageEnglish
Published National Academy of Sciences of the United States of America 28.03.1995
Subjects
Arteries
Bleeding time
Blood
Blood plasma
Bone marrow
Bone marrow transplantation
Physical trauma
Platelets
Swine
Thrombosis
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Summary:von Willebrand factor (vWF) is essential for the induction of occlusive thrombosis in stenosed and injured pig arteries and for normal hemostasis. To separate the relative contribution of plasma and platelet vWF to arterial thrombosis, we produced chimeric normal and von Willebrand disease pigs by crossed bone marrow transplantation; von Willebrand disease (vWD) pigs were engrafted with normal pig bone marrow and normal pigs were engrafted with vWD bone marrow. Thrombosis developed in the chimeric normal pigs that showed normal levels of plasma vWF and an absence of platelet vWF; but no thrombosis occurred in the chimeric vWD pigs that demonstrated normal platelet vWF and an absence of plasma vWF. The ear bleeding times of the chimeric pigs were partially corrected by endogenous plasma vWF but not by platelet vWF. Our animal model demonstrated that vWF in the plasma compartment is essential for the development of arterial thrombosis and that it also contributes to the maintenance of bleeding time and hemostasis.
ISSN:0027-8424
1091-6490