Hydrolysis and Reconjugation of Gibberellin A20Glucosyl Ester by Seedlings of Zea mays L

The [6-2H]glucosyl ester of [17-13C,3H]gibberellin A20(GA20) was injected into light-grown 14-day-old seedlings of normal, dwarf-1, and dwarf-5 maize (Zea mays L.). The plant material was extracted 24 h later, and the extracts were purified by solvent partitioning, column chromatography, and HPLC.13...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 89; no. 17; pp. 8045 - 8048
Main Authors Schneider, Gernot, Jensen, Einar, Spray, Clive R., Phinney, Bernard O.
Format Journal Article
LanguageEnglish
Published National Academy of Sciences of the United States of America 01.09.1992
Subjects
Corn
Esters
Gibberellins
Glucosides
Hydrolysis
Metabolites
Plant biochemistry
Plants
Radioactive decay
Seedlings
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Summary:The [6-2H]glucosyl ester of [17-13C,3H]gibberellin A20(GA20) was injected into light-grown 14-day-old seedlings of normal, dwarf-1, and dwarf-5 maize (Zea mays L.). The plant material was extracted 24 h later, and the extracts were purified by solvent partitioning, column chromatography, and HPLC.13C-labeled metabolites were identified from the purified extracts by full-scan gas chromatography/mass spectrometry and selected ion current monitoring in conjunction with Kovats retention indices. The metabolites, [13C]GA20, [13C]GA29, [13C]GA20-13-O-glucoside, and [13C]GA29-2-O-glucoside, were identified from normal, dwarf-1, and dwarf-5 seedlings. [13C]GA8and [13C]GA8-2-O-glucoside were also identified from normal and dwarf-5 seedlings but not from dwarf-1 seedlings. The data provide definitive evidence for the endogenous hydrolysis by the seedlings of the introduced conjugate and its reconjugation to three glucosides.
ISSN:0027-8424
1091-6490