Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens

This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day...

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Published inJournal of Veterinary Medical Science p. 20-0404
Main Authors NGUYEN, Yen Thi Hoang, NONAKA, Nariaki, MARUYAMA, Haruhiko, YOSHIDA, Ayako
Format Journal Article
LanguageEnglish
Published JAPANESE SOCIETY OF VETERINARY SCIENCE 2021
Subjects
Ascaris suum
chicken liver
real-time PCR assay
white spot lesion
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Summary:This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.20-0404