Recent technical advancements enabled atomic resolution CryoEMProject supported by Tsinghua-Peking Joint Center for Life Sciences, China

With recent breakthroughs in camera and image processing technologies single-particle electron cryo-microscopy (CryoEM) has suddenly gained the attention of structural biologists as a powerful tool able to solve the atomic structures of biological complexes and assemblies. Compared with x-ray crysta...

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Bibliographic Details
Published inChinese physics B Vol. 25; no. 1
Main Author Li, Xueming
Format Journal Article
LanguageEnglish
Published IOP Publishing 07.12.2015
Subjects
CryoEM
direct direction detector
dose fractionation
electron counting
three-dimensional classification
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Summary:With recent breakthroughs in camera and image processing technologies single-particle electron cryo-microscopy (CryoEM) has suddenly gained the attention of structural biologists as a powerful tool able to solve the atomic structures of biological complexes and assemblies. Compared with x-ray crystallography, CryoEM can be applied to partially flexible structures in solution and without the necessity of crystallization, which is especially important for large complexes and assemblies. This review briefly explains several key bottlenecks for atomic resolution CryoEM, and describes the corresponding solutions for these bottlenecks based on the recent technical advancements. The review also aims to provide an overview about the technical differences between its applications in biology and those in material science.
ISSN:1674-1056
DOI:10.1088/1674-1056/25/1/018710