Sensors

• Published in: Testing and Analysis of GMO-containing Foods and Feed pp. 155 - 204
• Main Authors: López-Ruiz, Beatriz, Sánchez-Paniagua López, Marta, Lorena Manzanares-Palenzuela, Carmen
• Format: Book Chapter
• Language: English
• Published: CRC Press 2019
• Edition: 1
• Subjects: FET Device; GMO Testing; DNA Capture Probe; Event Specific Methods; Roundup Ready Soybean; PCR Product; DNA Immobilization; Bare GCE; Field Effect Transistors; GMO Detection; SA Sensor Chip; Amplify DNA; DNA Probe; Target DNA; Optical Biosensors; GCE Surface; DNA Sensor; DNA Biosensor; GMO Content; Graphene Oxide; Immobilize DNA Probe; Electrode Surface; Capture Probe; Sensor Chip
• ISBN: 9781138036383; 1138036382
• DOI: 10.1201/9781315178592-5

An amplified electrical signal results from the interaction between some chemical species and the sensor". Biosensors are considered as a cutting-edge technology, as they allow the development of fast and inexpensive analytical methods with desirable properties, such as specificity, sensitivity, real-time analysis, ease of automation, and simplicity of operation. The fundamental basis of all immunosensors is the specific molecular recognition of immunogenic compounds by antibodies. The advent of low-cost synthetic oligonucleotides opened the door to the use of nucleic acids as recognition elements for affinity biosensors. Thermometric biosensors or thermistors are capable of measuring the variation in temperature of the biocatalytic reaction, which is directly related to the concentration of the analyte. Gold and carbon electrodes are among the most popular electrode materials employed in the development of DNA sensors. Most genosensors based on gene-specific detection use phosphinothricin acetyltransferase gene as target and include a screening procedure consisting on polymerase chain reaction amplification and detection of nitric oxide synthase gene.