Phytochemical investigation and biological activity of Doronicum pardalianches L. roots against Alzheimer's disease

The methanol extract of Doronicum pardalianches L. was fractionated using n-hexane, chloroform, and ethyl acetate to evaluate their cholinesterase (ChE) inhibitory activity via modified Ellman's method. It was perceived that only the ethyl acetate fraction was active toward acetylcholinesterase...

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Bibliographic Details
Published inNatural Product Research Vol. 37; no. 7; pp. 1227 - 1231
Main Authors Manayi, Azadeh, Rastegari, Arezoo, Heydarian, Bita, Vahedi-Mazdabadi, Yasaman, Khanavi, Mahnaz, Akbarzadeh, Tahmineh, Saeedi, Mina
Format Report
LanguageEnglish
Published Taylor & Francis 03.04.2023
Subjects
AChE
BChE
Doronicum pardalianches
metal chelating
neuroprotectivity
phytochemistry
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Summary:The methanol extract of Doronicum pardalianches L. was fractionated using n-hexane, chloroform, and ethyl acetate to evaluate their cholinesterase (ChE) inhibitory activity via modified Ellman's method. It was perceived that only the ethyl acetate fraction was active toward acetylcholinesterase (AChE) with IC 50 value of 172.21 µg/mL. Also, all fractions showed no butyrylcholinesterase (BChE) inhibitory activity. The ethyl acetate fraction was also investigated for its neuroprotectivity and metal chelating ability (Zn 2+ , Fe 2+ , and Cu 2+ ) which demonstrated desired activity. Phytochemical analysis of the ethyl acetate fraction led to isolation and identification of formononetin 7-O-β-D-glucopyranoside which has not been previously reported for this plant.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2021.1999944