Larvicidal activity of Brunfelsia uniflora extracts on Aedes aegypti larvae

• Published in: Natural Product Research Vol. 36; no. 4; pp. 1031 - 1037
• Main Authors: Sugauara, Elaine Yae Yamashita, Sugauara, ElisângelaYumi, Sugauara, Rosângela Rumi, Bortolucci, Wanessa de Campos, Fernandez, Carla Maria Mariano, Gonçalves, José Eduardo, Colauto, Nelson Barros, Gazim, Zilda Cristiani, Linde, Giani Andrea
• Format: Report
• Language: English
• Published: Taylor & Francis 16.02.2022
• Subjects: Aedes aegypti; bioinsecticide; Brunfelsia uniflora; phytol; α-amyrin
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2020.1844685

The aim of this study was to evaluate the chemical composition and the larvicidal activity of Brunfelsia uniflora leaf and flower extracts against Aedes aegypti larvae. Twenty-four compounds were found in the leaf extract, and the major compounds were phytol (23.1%), 9,12,15-octadecatrienoic acid, ethyl ester (21.3%), and hexadecanoic acid, ethyl ester (12.8%). In the flower extract, twenty-four compounds were also identified and the major compounds were α-amyrin (35.7%), β-amyrin (16.4%), and (EE)-geranyl linalool (9.6%) by gas chromatography coupled to mass spectrometry. The larvicidal activity was evaluated by larval immersion test. The lethal concentrations (LC) obtained from leaf extract were LC 50 = 4.89 and LC 99.9 = 11.14 mg/mL and from flower extract were LC 50 = 3.82 and LC 99.9 = 11.03 mg/mL, and the positive control presented LC 50 = 0.40 and LC 99.9 = 1.14 mg/mL. Thus, B. uniflora extracts are promising alternatives to control A. aegypti larvae.