Amplificación múltiple de ADN para la detección de Escherichia coli O157:H7 y Salmonella spp. en canales de bovino Multiplex DNA amplification to detect Escherichia coli O157:H7 and Salmonella spp. in bovine carcasses

• Published in: CYTA: journal of food Vol. 7; no. 1; pp. 31 - 36
• Main Authors: García López, E., Rubio Lozano, Ma. Salud, Alonso Morales, R. A., Gayosso Vazquez, A., Miranda Castro, S. P., Nicoli Tolosa, M., Núñez Espinosa, J. F.
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 01.05.2009
• Subjects: biological contamination; canal; carcasses; contaminación biológica; Escherichia coli O157:H7; PCR; Salmonella spp

The objective of this work was to develop a polymerase chain reaction (PCR) multiplex technology to detect Salmonella spp. and Escherichia coli O157:H7 in bovine carcasses. To obtain the PCR products, slt-I, slt-II, eaeA and uidA for E. coli and invA for Salmonella spp. genes were amplified in the s...