Amplificación múltiple de ADN para la detección de Escherichia coli O157:H7 y Salmonella spp. en canales de bovino Multiplex DNA amplification to detect Escherichia coli O157:H7 and Salmonella spp. in bovine carcasses

The objective of this work was to develop a polymerase chain reaction (PCR) multiplex technology to detect Salmonella spp. and Escherichia coli O157:H7 in bovine carcasses. To obtain the PCR products, slt-I, slt-II, eaeA and uidA for E. coli and invA for Salmonella spp. genes were amplified in the s...

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Bibliographic Details
Published inCYTA: journal of food Vol. 7; no. 1; pp. 31 - 36
Main Authors García López, E., Rubio Lozano, Ma. Salud, Alonso Morales, R. A., Gayosso Vazquez, A., Miranda Castro, S. P., Nicoli Tolosa, M., Núñez Espinosa, J. F.
Format Journal Article
LanguageEnglish
Published Taylor & Francis 01.05.2009
Subjects
biological contamination
canal
carcasses
contaminación biológica
Escherichia coli O157:H7
PCR
Salmonella spp
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Summary:The objective of this work was to develop a polymerase chain reaction (PCR) multiplex technology to detect Salmonella spp. and Escherichia coli O157:H7 in bovine carcasses. To obtain the PCR products, slt-I, slt-II, eaeA and uidA for E. coli and invA for Salmonella spp. genes were amplified in the same reaction. Using this method, microbial presence was investigated in carcasses from Mexican slaughterhouses, and traditional microbiological methods were used to corroborate PCR technology. Sixty samples were collected from municipal slaughterhouses and 60 from federal inspected slaughterhouses (TIF for its initials in Spanish) after sacrifice. Two positive samples (1.6%) were obtained of Salmonella spp. using both techniques in the municipal slaughterhouses. In the samples from both origins, there were positives. The PCR technique proved effective for the rapid and simultaneous identification of microorganisms.
ISSN:1947-6337
1947-6345
DOI:10.1080/11358120902850651