Phylogenetic analysis of bovine Leptospira isolates

Leptospira isolates obtained from bovines were subjected to phylogenetic analysis by sequencing 16s rRNA gene of Leptospira. The phylogenetic analysis of the 16s rRNA of 9 isolates were grouped into three clusters. Partial sequence analysis of 16s rRNA of the isolates revealed that they were having...

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Bibliographic Details
Published inIndian journal of field veterinarians Vol. 10; no. 3; pp. 36 - 39
Main Authors Balakrishnan, G, Roy, Parimal, Kirubaharan, J. John, Meenambigai, T.V, Chandran, N. Daniel Joy
Format Journal Article
LanguageEnglish
Published Academa Publishers 01.01.2015
Subjects
Bovines
Leptospira
PCR
Phylogenetic analysis
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Summary:Leptospira isolates obtained from bovines were subjected to phylogenetic analysis by sequencing 16s rRNA gene of Leptospira. The phylogenetic analysis of the 16s rRNA of 9 isolates were grouped into three clusters. Partial sequence analysis of 16s rRNA of the isolates revealed that they were having 99.30 to 100.00 per cent identity among the australis isolates with reference strain, Australis australis Ballico. The Leptospira ballum isolate showed 99.70 per cent identity with reference strain and also encompassed with Leptospira hebdomadis isolates and some of the Leptospira australis isolates.
ISSN:2394-0247
0976-1918