Integration of "on-chip" electrochemical detection in a microfabricated capillary electrophoresis device

Our efforts have been focused on developing a self-contained and transportable capillary electrophoresis (CE) system with integrated electrochemical detection (ECD). The current prototype includes all necessary electrodes "on-chip" and utilizes miniaturized CE and ECD supporting electronic...

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Published inProceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology Vol. 2; pp. 1640 - 1641 vol.2
Main Authors Roussel, T.J., Crain, M.M., Bathlagundu, V., Jackson, D.J., Conklin, J.A., Pai, R., Naber, J.F., Walsh, K.M., Gullapalli, J., Baldwin, R.P., Keynton, R.S.
Format Conference Proceeding
LanguageEnglish
Published IEEE 2002
Subjects
Bonding
Chemicals
Electrodes
Electrokinetics
Etching
Glass
Microchannel
Performance analysis
Platinum
Prototypes
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Summary:Our efforts have been focused on developing a self-contained and transportable capillary electrophoresis (CE) system with integrated electrochemical detection (ECD). The current prototype includes all necessary electrodes "on-chip" and utilizes miniaturized CE and ECD supporting electronics custom designed for this purpose. A novel microfabrication procedure was used to create recessed platinum electrodes and the CE/EC device from two patterned ultra-flat glass substrates. The electrodes in the bottom substrate were formed by a self-aligned etch and deposition technique followed by a photolithographic lift-off process. The microchannels (20 /spl mu/m deep/spl times/50 /spl mu/m wide [avg]) were chemically etched into the top substrate followed by thermal bonding to complete the microchip device. CE/EC experiments were performed using 0.02M phosphate buffer (pH 6), an analyte/buffer solution (2.2 mM dopamine, 2.3 mM catechol) and varying separation voltages (0-250 V) with a custom electronics unit interfaced to a laptop computer for data acquisition. Detection limits (S/N=3) were found to be 4.3 /spl mu/M for dopamine and 4.4 /spl mu/M for catechol. A linear detection response was observed up to 2200 /spl mu/M and 2300 /spl mu/M for dopamine and catechol, respectively.
ISBN:0780376129
9780780376120
ISSN:1094-687X
1558-4615
DOI:10.1109/IEMBS.2002.1106578