Utilizing bioinformatics approaches for identifying binding sites in the Drosophila genome for a developmentally important transcription factor protein

Regulation of gene expression, at the transcription level, in eukaryotes is largely dependent on regulatory proteins called transcription factors. Transcription factors bind to highly specific, short nucleotide sequences on the DNA and regulate transcription by facilitating or inhibiting the binding...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology Vol. 3; pp. 2186 - 2187 vol.3
Main Authors Patel, H.B., Lee, J.
Format Conference Proceeding
LanguageEnglish
Published IEEE 2002
Subjects
Biochemistry
Bioinformatics
DNA
Functional analysis
Gene expression
Genomics
Polymers
Protein engineering
RNA
Sequences
Online AccessGet full text

Cover

More Information
Summary:Regulation of gene expression, at the transcription level, in eukaryotes is largely dependent on regulatory proteins called transcription factors. Transcription factors bind to highly specific, short nucleotide sequences on the DNA and regulate transcription by facilitating or inhibiting the binding of RNA polymerase II, the enzyme that catalyzes transcription of eukaryote genes. We are using computational tools that use inherent pattern-searching algorithms to identify binding sites for the Stripe protein, an important transcription factor in Drosophila development. The binding sites for Stripe are expected to lie close to genes that are known to be regulated by this transcription factor. Identifying the locations of these binding sites can be useful in determining the biological significance of these sites through further functional analyses.
ISBN:0780376129
9780780376120
ISSN:1094-687X
1558-4615
DOI:10.1109/IEMBS.2002.1053231