Automated Large-Scale Spheroid Generation via Hanging-Drop and Efficient Transfer into Physiological Mimicking Microenvironment

We present a high-throughput three-dimensional (3D) cell culture workflow combining fully automated large-scale production of spheroids with an automated transfer into microwells loaded with an extracellular matrix mimicking hydrogel. The platform principle is based on dispensing nanoliter droplets...

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Bibliographic Details
Published in2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) pp. 650 - 653
Main Authors Zieger, Viktoria, Woehr, Ellen, Zimmermann, Stefan, Frejek, Daniel, Koltay, Peter, Zengerle, Roland, Kartmann, Sabrina
Format Conference Proceeding
LanguageEnglish
Published IEEJ 25.06.2023
Subjects
3D In Vitro Model
Hydrogels
Large-scale Spheroid Generation
Micromechanical devices
Physiology
Production
Solid modeling
Spheroid Harvesting
Three-dimensional displays
Transducers
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Summary:We present a high-throughput three-dimensional (3D) cell culture workflow combining fully automated large-scale production of spheroids with an automated transfer into microwells loaded with an extracellular matrix mimicking hydrogel. The platform principle is based on dispensing nanoliter droplets of a homogeneously mixed cell suspension with a non-contact dispenser so that spheroids are generated via the hanging-drop method. Compared to previous work [1], [2] we optimized the setup for extended sample processing times of up to 45 minutes without negatively affecting the quality of spheroid generation. We reached a superior spheroid yield of 99.3 % while simultaneously generating highly consistent spheroids with a coefficient of variance below 8 %. Our novel harvesting workflow based on centrifugal drop transfer dramatically increased the efficiency to 100 % compared to previously reported 54.3 % [1].
ISSN:2167-0021