Implantable Biosensor for Continuous Serotonin Detection in Freely Moving Crayfish

This work presents, for the first time, an implantable biosensor for continuous in vivo serotonin (5-HT) monitoring in freely moving crayfish. 5-HT is an important neurotransmitter that regulates animal behaviors. Conventional methods for detecting 5-HT do not provide real-time information. Our appr...

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Bibliographic Details
Published in2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) pp. 1860 - 1863
Main Authors Han, Jinjing, Ho, Tawen, Stine, Justin M., Straker, Michael A., Herberholz, Jens, Ghodssi, Reza
Format Conference Proceeding
LanguageEnglish
Published IEEJ 25.06.2023
Subjects
Biosensing
Electrochemical Device
Electrochemistry
Implantable Electrode
In vivo
In Vivo Sensing
Real-time systems
Sensitivity
Sensors
Solid modeling
Transducers
Wireless communication
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Summary:This work presents, for the first time, an implantable biosensor for continuous in vivo serotonin (5-HT) monitoring in freely moving crayfish. 5-HT is an important neurotransmitter that regulates animal behaviors. Conventional methods for detecting 5-HT do not provide real-time information. Our approach facilitates continuous electrochemical monitoring of 5-HT levels without impeding animal natural behavior. The sensing system shows a linear range of 0.4 - 1.2 μΜ with a sensitivity of 0.07 μΑ/μΜ for 5-HT detection. With a 3D-printed package, the sensor can be easily attached to crayfish dorsal carapace allowing electrodes implantation into crayfish heart to monitor neurohormonal dynamics of 5-HT. The implantable biosensor system displayed a significant increase in oxidation currents after 5-HT injections. The device successfully demonstrates the application for in vivo detection of 5-HT in the hemolymph (i.e., blood) of freely behaving crayfish underwater.
ISSN:2167-0021