二苯甲醯基甲烷經由小鼠肝臟微粒體酵素作用之代謝途徑研究

• Published in: 藥物食品分析 Vol. 13; no. 3; pp. 284 - 288
• Main Authors: 林娟娟(Lin, Chuan-chuan), 魏國晉(Wei, Guor-jien), 黃茂端(Huang, Mou-tuan), 何其儻(Ho, Chi-tang)
• Format: Journal Article
• Language: Chinese
• Published: 台灣 衛生福利部食品藥物管理署 01.09.2005
• Subjects: 7,12-dimethylbenz[a]anthracene; 7,12-二甲基苯并蔥; Dibenzoylmethane; Mammary tumorigenesis; MEDLINE; Mouse liver microsomes; NADPH-dependent cytochrome P450 enzymes; NADPH-相關細胞色素代謝酵素; Reductive metabolite; SCIE; Scopus; 二苯甲醯基甲烷; 小鼠肝臟微粒體; 還原型代謝產物
• ISSN: 1021-9498

Dibenzoylmethane (DBM), a curcumin-related β-diketone analogue, has been reported to exhibit a remarkable inhibitory effect on 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumorigenesis in Sencar mice. Investigation of the underlying mechanisms of DBM in the prevention of mammary tumorigenesis implied its role as an effector of Phase I enzymatic system. In this report, the metabolic fate of DBM by NADPH-dependent cytochrome P450 enzymes in mouse liver microsomes was demonstrated. Isolation of the major reductive metabolites of DBM (DBMH2), together with several minor metabolites identified by NMR, GC and LC-MS, explained the potential role of DBM as a modulator of the cytochrome P450 reductase that is required for the function of oxidase to metabolize DMBA. These might also contribute to the result of the inhibitory effect of DBM on DMBA-induced mouse mammary tumorigenesis.
二苯甲醯基甲烷（DBM）為一薑黃素之β-二酮類結構類似物，從以往本實驗室研究結果顯示DBM可有效抑制7,12-二甲基苯并蔥（DMBA）誘發之小鼠乳腺腫瘤。研究其中之作用機制顯示DBM可能扮演肝臟代謝酵素Phase I系統中之調節劑。此報告中，我們分析DBM經