高效液相層析法檢測血清中9,10-蔥醌酮-2-羧酸濃度及其在藥物動力學研究之應用

• Published in: 藥物食品分析 Vol. 7; no. 3; pp. 199 - 205
• Main Authors: 鄭信宏(Cheng, Hshin-hrong), 林文川(Lin, Wen-chuan), 黃太鴻(Huang, Tai-hung), 郭盛助(Kuo, Sheng-chu), 蔡尚元(Tsai, Shang-yuan)
• Format: Journal Article
• Language: Chinese
• Published: 台灣 衛生福利部食品藥物管理署 01.09.1999
• Subjects: 9,10-anthraquinone-2-carboxylic acid; 9,10-蔥醌酮-2-羧酸; Bioavailability; High performance liquid chromatography; HPLC; MEDLINE; Pharmacokinetics; SCIE; Scopus; 生體可用率; 藥物動力學; 高效液相層析法
• ISSN: 1021-9498

A simple and sensitive high-performance liquid chromatographic (HPLC) methodinvolving UV detection was developed for the determination of 9,10-anthraquinone-2-carboxylic acid (AQCA) in rabbit serum. A reverse-phase column(Lichrosher R 100RP-18) was eluted with a mobile phase of 0.4% phosphoric acid:acetonitrile (7:3) / methanol = 45 : 55 at a flow rate of 1.2 mL/min. The UV absorbancewas monitored at 256 nm. After a simple clean-up procedure, the limit of quantitationachieved was 0.6 μg/mL and the standard curve was found to be linear over the serumconcentration of 0.6-18μg/mL. The intra-assay and inter-assay coefficient ofvariance in serum was less than 2%, and the recover v was 96.98%.bioavailability of 9,10-anthraquinone-2-carboxylic acid (AQCA). Eighteen healthyrabbits were divided into three groups and given intravenous (i.v.), oral and rectaladministrations of different AQCA preparations with a single dose of 10 mg/kg. Bloodsamples were collected and AQCA concentrations in serum were analyzed. The resul