Moraxella catarrhalis induces inflammatory response of bronchial epithelial cells via MAPK and NF-{kappa}B activation and histone deacetylase activity reduction
Departments of 1 Internal Medicine/Infectious Diseases and 2 Periodontology and Synoptic Dentistry, Charité-Universitätsmedizin Berlin, Berlin, Germany Submitted 6 October 2005 ; accepted in final form 23 December 2005 Moraxella catarrhalis is a major cause of infectious exacerbations of chronic obs...
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Published in | American journal of physiology. Lung cellular and molecular physiology Vol. 290; no. 5; p. L818 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.05.2006
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Online Access | Get full text |
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Summary: | Departments of 1 Internal Medicine/Infectious Diseases and 2 Periodontology and Synoptic Dentistry, Charité-Universitätsmedizin Berlin, Berlin, Germany
Submitted 6 October 2005
; accepted in final form 23 December 2005
Moraxella catarrhalis is a major cause of infectious exacerbations of chronic obstructive lung disease (COPD) and may also contribute to the pathogenesis of COPD. Little is known about M. catarrhalis -bronchial epithelium interaction. We investigated activation of M. catarrhalis infected bronchial epithelial cells and characterized the signal transduction pathways. Moreover, we tested the hypothesis that the M. catarrhalis -induced cytokine expression is regulated by acetylation of histone residues and controlled by histone deacetylase activity (HDAC). We demonstrated that M. catarrhalis induced a strong time- and dose-dependent inflammatory response in the bronchial epithelial cell line (BEAS-2B), characterized by the release of IL-8 and GM-CSF. For this cytokine liberation activation of the ERK and p38 mitogen-activated protein (MAP) kinases and transcription factor NF- B was required. Furthermore, M. catarrhalis -infected bronchial epithelial cells showed an enhanced acetylation of histone H3 and H4 globally and at the promoter of the il8 gene. Preventing histone deacetylation by the histone deacetylase inhibitor trichostatin A augmented the M. catarrhalis -induced IL-8 response. After exposure to M. catarrhalis , we found a decrease in global histone deacetylase expression and activity. Our findings suggest that M. catarrhalis -induced activation of il8 gene transcription was caused by interference with epigenetic mechanisms regulating il8 gene accessibility. Our findings provide insight into important molecular and cellular mechanisms of M. catarrhalis -induced activation of human bronchial epithelium.
chronic obstructive pulmonary disease; bronchial epithelium; immune response; mitogen-activated protein kinase; nuclear factor- B
Address for reprint requests and other correspondence: H. Slevogt, Dept. of Internal Medicine/Infectious Diseases, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany (e-mail: hortense.slevogt{at}charite.de ) |
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ISSN: | 1040-0605 1522-1504 |
DOI: | 10.1152/ajplung.00428.2005 |