Estrogen Receptor α and the Activating Protein-1 Complex Cooperate during Insulin-like Growth Factor-I-induced Transcriptional Activation of the pS2/TFF1 Gene
Insulin like growth factor I (IGF-I) displays estrogenic activity in breast cancer cells. This activity is strictly dependent on the presence of estrogen receptor α (ERα). However the precise molecular mechanisms involved in this process are still unclear. IGF-I treatment induces phosphorylation o...
Saved in:
Published in | The Journal of biological chemistry Vol. 282; no. 16; p. 11732 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
American Society for Biochemistry and Molecular Biology
20.04.2007
|
Online Access | Get full text |
Cover
Loading…
Summary: | Insulin like growth factor I (IGF-I) displays estrogenic activity in breast cancer cells. This activity is strictly dependent
on the presence of estrogen receptor α (ERα). However the precise molecular mechanisms involved in this process are still
unclear. IGF-I treatment induces phosphorylation of the AF1 domain of ERα and activation of estrogen regulated genes. These
genes are characterized by important differences in promoter architecture and response element composition. We show that promoter
structure is crucial for IGF-I-induced transcription activation. We demonstrate that on a complex promoter such as the pS2/TFF1
promoter, which contains binding sites for ERα and for the activating protein-1 (AP1) complex, transcriptional activation
by IGF-I requires both ERα and the AP1 complex. IGF-I is unable to stimulate transcription of an estrogen-regulated gene under
the control of a minimal promoter containing only a binding site for ERα. We propose a molecular mechanism with stepwise assembly
of the AP1 complex and ERα during transcription activation of pS2/TFF1 by IGF-I. IGF-I stimulation induces rapid phosphorylation
and an increase in protein levels of the AP1 complex. Binding of the phosphorylated AP1 complex to the pS2/TFF1 promoter allows
recruitment of the chromatin remodeling factor Brg1 followed by binding of ERα via its interaction with c-Jun. |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M610079200 |