RNA Silencing Identifies PDE4D5 as the Functionally Relevant cAMP Phosphodiesterase Interacting with βArrestin to Control the Protein Kinase A/AKAP79-mediated Switching of the β2-Adrenergic Receptor to Activation of ERK in HEK293B2 Cells

• Published in: The Journal of biological chemistry Vol. 280; no. 39; p. 33178
• Main Authors: Martin J. Lynch, George S. Baillie, Ahmed Mohamed, Xiang Li, Charlotte Maisonneuve, Enno Klussmann, Gino van Heeke, Miles D. Houslay
• Format: Journal Article
• Language: English
• Published: American Society for Biochemistry and Molecular Biology 30.09.2005
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M414316200

PDE4B and PDE4D provide >90% of PDE4 cAMP phosphodiesterase activity in human embryonic kidney (HEK293B2) cells. Their selective small interference RNA (siRNA)-mediated knockdown potentiates isoprenaline-stimulated protein kinase A (PKA) activation. Whereas endogenous PDE4D co-immunoprecipitates with βarrestin, endogenous PDE4B does not, even upon PDE4D knockdown. Ectopic overexpression of PDE4B2 confers co-immunoprecipitation with βarrestin. Knockdown of PDE4D, but not PDE4B, amplifies isoprenaline-stimulated phosphorylation of the β 2 -adrenergic receptor (β 2 -AR) by PKA and activation of extracellular signal-regulated kinase (ERK) through G i . Isoform-selective knockdown identifies PDE4D5 as the functionally important species regulating isoprenaline stimulation of both these processes. Ht31-mediated disruption of the tethering of PKA to AKAP scaffold proteins attenuates isoprenaline activation of ERK, even upon PDE4D knockdown. Selective siRNA-mediated knockdown identifies AKAP79, which is constitutively associated with the β 2 -AR, rather than isoprenaline-recruited gravin, as being the functionally relevant AKAP in this process. Isoprenaline-stimulated membrane recruitment of PDE4D is ablated uponβarrestin knockdown. A mutation that compromises interactions with βarrestin prevents catalytically inactive PDE4D5 from performing a dominant negative role in potentiating isoprenaline-stimulated ERK activation. βarrestin-recruited PDE4D5 desensitizes isoprenaline-stimulated PKA phosphorylation of the β 2 -AR and the consequential switching of its signaling to ERK. The ability to observe a cellular phenotype upon PDE4D5 knockdown demonstrates that other PDE4 isoforms, expressed at endogenous levels, are unable to afford rescue in HEK293B2 cells.