Disruption of Pancreatic β-Cell Lipid Rafts Modifies Kv2.1 Channel Gating and Insulin Exocytosis
In pancreatic β-cells, the predominant voltage-gated Ca 2+ channel (Ca V 1.2) and K + channel (K V 2.1) are directly coupled to SNARE (soluble N -ethylmaleimide-sensitive factor attachment protein (SNAP) receptor) proteins. These SNARE proteins modulate channel expression and gating and closely ass...
Saved in:
Published in | The Journal of biological chemistry Vol. 279; no. 23; p. 24685 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
American Society for Biochemistry and Molecular Biology
04.06.2004
|
Online Access | Get full text |
Cover
Loading…
Summary: | In pancreatic β-cells, the predominant voltage-gated Ca 2+ channel (Ca V 1.2) and K + channel (K V 2.1) are directly coupled to SNARE (soluble N -ethylmaleimide-sensitive factor attachment protein (SNAP) receptor) proteins. These SNARE proteins modulate channel expression
and gating and closely associate these channels with the insulin secretory vesicles. We show that K V 2.1 and Ca V 1.2, but not K V 1.4, SUR1, or Kir6.2, target to specialized cholesterol-rich lipid raft domains on β-cell plasma membranes. Similarly, the
SNARE proteins syntaxin 1A, SNAP-25, and VAMP-2, but not Munc-13-1 or n-Sec1, are associated with lipid rafts. Disruption
of the lipid rafts by depleting membrane cholesterol with methyl-β-cyclodextrin shunts K V 2.1, Ca V 1.2, and SNARE proteins out of lipid rafts. Furthermore, methyl-β-cyclodextrin inhibits K V 2.1 but not Ca V 1.2 channel activity and enhances single-cell exocytic events and insulin secretion. Membrane compartmentalization of ion
channels and SNARE proteins in lipid rafts may be critical for the temporal and spatial coordination of insulin release, forming
what has been described as the excitosome complex. |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M314314200 |