Full Activation of Estrogen Receptor α Activation Function-1 Induces Proliferation of Breast Cancer Cells

• Published in: The Journal of biological chemistry Vol. 278; no. 29; p. 26704
• Main Authors: Tetsuo Fujita, Yoko Kobayashi, Osamu Wada, Yukiyo Tateishi, Lina Kitada, Yasuji Yamamoto, Hisashige Takashima, Akiko Murayama, Tetsu Yano, Tadashi Baba, Shigeaki Kato, Yoh-ichi Kawabe, Junn Yanagisawa
• Format: Journal Article
• Language: English
• Published: American Society for Biochemistry and Molecular Biology 18.07.2003
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M301031200

The effects of estrogen and anti-estrogen are mediated through the estrogen receptors (ER) α and β, which function as ligand-induced transcriptional factors. Recently, one of the phthalate esters, n -butylbenzyl phthalate (BBP), has been shown to induce estrogen receptor-mediated responses. By using the truncated types of ER mutants, we revealed that activation function-1 (AF-1) activity was necessary for the BBP-dependent transactivation function of ERα. AF-1 is also known to be responsible for the partial agonistic activity of tamoxifen. Whereas tamoxifen exhibits an anti-estrogenic effect on proliferation of the MCF-7 breast cancer cell line, BBP showed an estrogenic effect on MCF-7 to stimulate proliferation. In vivo and in vitro binding assays revealed that whereas 4-hydroxytamoxifen (OHT) induced binding of ERα to both an AF-1 coactivator complex (p68/p72 and p300) and corepressor complexes (N-CoR/SMRT), BBP selectively enhanced the binding to the AF-1 coactivators. We also showed that the transcriptional activity of OHT-bound ERα was modulated by the ratio between the AF-1 coactivator and corepressor complexes. Expression of a dominant-negative type of N-CoR inhibited the interaction between OHT-bound ERα and N-CoR/SMRT and enhanced the transcriptional activity of OHT-bound ERα. Furthermore, the cell growth of MCF-7 stably expressing the dominant-negative type of N-CoR was enhanced by the addition of OHT. These results indicated that fully activated AF-1 induces the stimulation of breast cancer growth and that the ratio between AF-1 coactivators and corepressors plays a key role to prevent proliferation of tumor by tamoxifen.