Upstream Elements Present in the 3â²-Untranslated Region of Collagen Genes Influence the Processing Efficiency of Overlapping Polyadenylation Signals
3â²-Untranslated regions (UTRs) of genes often contain key regulatory elements involved in gene expression control. A high degree of evolutionary conservation in regions of the 3â²-UTR suggests important, conserved elements. In particular, we are interested in those elements involved in regulation...
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Published in | The Journal of biological chemistry Vol. 277; no. 45; p. 42733 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
American Society for Biochemistry and Molecular Biology
08.11.2002
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Online Access | Get full text |
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Summary: | 3â²-Untranslated regions (UTRs) of genes often contain key regulatory elements involved in gene expression control. A high
degree of evolutionary conservation in regions of the 3â²-UTR suggests important, conserved elements. In particular, we are
interested in those elements involved in regulation of 3â² end formation. In addition to canonical sequence elements, auxiliary
sequences likely play an important role in determining the polyadenylation efficiency of mammalian pre-mRNAs. We identified
highly conserved sequence elements upstream of the AAUAAA in three human collagen genes, COL1A1, COL1A2, and COL2A1, and demonstrate
that these upstream sequence elements (USEs) influence polyadenylation efficiency. Mutation of the USEs decreases polyadenylation
efficiency both in vitro and in vivo , and inclusion of competitor oligoribonucleotides representing the USEs specifically inhibit polyadenylation. We have also
shown that insertion of a USE into a weak polyadenylation signal can enhance 3â² end formation. Close inspection of the COL1A2
3â²-UTR reveals an unusual feature of two closely spaced, competing polyadenylation signals. Taken together, these data demonstrate
that USEs are important auxiliary polyadenylation elements in mammalian genes. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M208070200 |