Cloning the cDNA for a New Human Zinc Finger Protein Defines a Group of Closely Related Krüppel-like Transcription Factors

• Published in: The Journal of biological chemistry Vol. 273; no. 43; p. 28229
• Main Authors: Nobukyuki Matsumoto, Friedrich Laub, Rafael Aldabe, Wen Zhang, Francesco Ramirez, Teruhiko Yoshida, Masaaki Terada
• Format: Journal Article
• Language: English
• Published: American Society for Biochemistry and Molecular Biology 23.10.1998
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.273.43.28229

We have identified a novel zinc finger protein that has been named ubiquitous Krüppel-like factor (UKLF) based on structural considerations and the pattern of gene expression. UKLF was isolated by the polymerase chain reaction approach using degenerate oligonucleotides corresponding to the DNA-binding domain of erythroid Krüppel-like factor (EKLF) and cDNA prepared from human vascular endothelial cells. The carboxyl-terminal portion of UKLF contains three zinc fingers of the Cys 2 -His 2 type and binds in vitro to the CACCC motif of the β-globin promoter and to the Sp1 recognition sequence. The amino-terminal portion of UKLF consists of a hydrophobic region rich in serines and a negatively charged segment with several glutamic acid residues. The first 47 amino acids of the acidic region are nearly identical to the amino-terminal portion of another Krüppel-like factor, the so-called core promoter-binding protein (CPBP) or Zf9. Like CPBP/Zf9, UKLF can function as a transcription activator in co-transfection assays. However, this activity is lost when the highly conserved amino-terminal segment is deleted. These findings indicate that UKLF and CPBP/Zf9 represent a distinct subgroup of closely related Krüppel-like activators of transcription. Mapping of the UKLF gene to chromosome 2 suggested that UKLF and CPBP/Zf9 translocated to different chromosomes following duplication from an ancestral gene.