Daunorubicin Activates NFκB and Induces κB-dependent Gene Expression in HL-60 Promyelocytic and Jurkat T Lymphoma Cells
The anthracycline antibiotic, daunorubicin, can induce programmed cell death (apoptosis) in cells. Recent work suggests that this event is mediated by ceramide via enhanced ceramide synthase activity. Since the generation of ceramide has been directly linked with the activation of the transcription...
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Published in | The Journal of biological chemistry Vol. 272; no. 20; p. 12952 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
American Society for Biochemistry and Molecular Biology
16.05.1997
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Online Access | Get full text |
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Summary: | The anthracycline antibiotic, daunorubicin, can induce programmed cell death (apoptosis) in cells. Recent work suggests that
this event is mediated by ceramide via enhanced ceramide synthase activity. Since the generation of ceramide has been directly
linked with the activation of the transcription factor, NFκB, this was investigated as a novel target for the action of daunorubicin.
Here we describe how treatment of HL-60 promyelocytes and Jurkat T lymphoma cells with daunorubicin results in the activation
of the transcription factor NFκB. The effect of daunorubicin was evident following 1â2 h treatment, which was in contrast
to the time course of activation obtained with the cytokine, tumor necrosis factor, where NFκB activation was detected within
minutes of cellular stimulation. Activated complexes were shown to contain predominantly p50 and p65/RelA subunit components.
Daunorubicin also induced IκB degradation and increased the expression of an NFκB-linked reporter gene. In addition, the drug
was found to strongly potentiate the ability of tumor necrosis factor to induce an NFκB-linked reporter gene, suggesting a
synergy between these two agents in this response. These events were sensitive to the iron chelator, deferoxamine mesylate
(desferal), and the anti-oxidant and metal chelator pyrrolidine dithiocarbamate. A structurally related compound, mitoxantrone,
which, unlike daunorubicin, is unable to undergo redox cycling in cells, also activated NFκB in a pyrrolidine dithiocarbamate-sensitive
manner. A specific inhibitor of ceramide synthase, fumonisin B1, had no effect on daunorubicin induced NFκB activation at
a range of concentrations previously reported to block apoptosis induced by this drug. However, this agent could inhibit increases
in ceramide induced by daunorubicin, in addition to blocking ceramide synthase activity from HL-60 cells which was activated
in response to daunorubicin treatment. These data therefore suggest that the effect of daunorubicin on NFκB is unlikely to
involve ceramide, but may involve reactive oxygen species generated as a result of endogenous cellular processes rather than
reductive metabolism of the drug. As NFκB may be involved in apoptosis, this effect may be an important aspect of the cellular
responses to this agent. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.272.20.12952 |