Molecular Cloning of Rodent p72

• Published in: The Journal of biological chemistry Vol. 270; no. 21; p. 12659
• Main Authors: R. Bruce Rowley, Joseph B. Bolen, Joseph Fargnoli
• Format: Journal Article
• Language: English
• Published: American Society for Biochemistry and Molecular Biology 26.05.1995
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.270.21.12659

Northern blot analysis of polyadenylated RNA prepared from RBL-2H3 cells revealed the presence of three distinct mRNAs encoding p72 , a protein-tyrosine kinase previously shown to be associated with the high affinity IgE receptor present on the surface of these cells (Hutchcroft, J. E., Geahlen, R. L., Deanin, G. G., and Oliver, J. M.(1992) Proc. Natl. Acad. Sci. U. S.A. 89, 9107-9111). Here we report the full-length nucleotide sequence of two of these messages, as well as the complete predicted amino acid sequence of the rodent p72 protein-tyrosine kinase. In addition, we report evidence indicating alternative splicing of p72 mRNAs within RBL-2H3 cells. This splicing event results in the expression of two distinct protein isoforms that differ with respect to the presence of a 23-amino acid insert located within the region of the protein that separates the two SH2 domains from the catalytic domain. Both mRNAs arising from this splicing event appear to encode functional protein-tyrosine kinases, as expression of the corresponding cDNAs in COS cells results in the production of proteins of the expected sizes that possess intrinsic tyrosine specific kinase activity.