Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism

BACKGROUND/AIMS: Polyunsaturated fatty acids (PUFA) deficiency is common in patients with alcoholic liver disease. The suitability of reversing such deficiency remains controversial. The aim was to investigate the role played by PUFA deficiency in the occurrence of alcohol-related mitochondrial dysf...

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Bibliographic Details
Published inJournal of hepatology Vol. 41; no. 5; pp. 721 - 9
Main Authors Piquet, Marie-Astrid, Roulet, Michel, Nogueira, Véronique, Filippi, Céline, Sibille, Brigitte, Hourmand-Ollivier, Isabelle, Pilet, Marianne, Rouleau, Vincent, Leverve, Xavier M
Format Journal Article
LanguageEnglish
Published Elsevier 01.11.2004
Subjects
Animal Feed
Animals
Dietary Fats
Energy Metabolism
Fatty Acids, Unsaturated
Liver Diseases, Alcoholic
Male
Mitochondria, Liver
Oxygen Consumption
Rats
Rats, Wistar
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Summary:BACKGROUND/AIMS: Polyunsaturated fatty acids (PUFA) deficiency is common in patients with alcoholic liver disease. The suitability of reversing such deficiency remains controversial. The aim was to investigate the role played by PUFA deficiency in the occurrence of alcohol-related mitochondrial dysfunction. METHODS: Wistar rats were fed either a control diet with or without alcohol (control and ethanol groups) or a PUFA deficient diet with or without alcohol (PUFA deficient and PUFA deficient+ethanol groups). After 6 weeks, liver mitochondria were isolated for energetic studies and fatty acid analysis. RESULTS: Mitochondria from ethanol fed rats showed a dramatic decrease in oxygen consumption rates and in cytochrome oxidase activity. PUFA deficiency showed an opposite picture. PUFA deficient+ethanol group roughly reach control values, regarding cytochrome oxidase activity and respiratory rates. The relationship between ATP synthesis and respiratory rate was shifted to the left in ethanol group and to the right in PUFA-deficient group. The plots of control and PUFA deficient+ethanol groups were overlapping. Phospholipid arachidonic over linoleic ratio closely correlated to cytochrome oxidase and oxygen uptake. CONCLUSIONS: PUFA deficiency reverses alcohol-related mitochondrial dysfunction via an increase in phospholipid arachidonic over linoleic ratio, which raises cytochrome oxidase activity. Such deficiency may be an adaptive mechanism.
ISSN:0168-8278
1600-0641
DOI:10.1016/j.jhep.2004.07.002