Anti-bis(monoacylglycero)phosphate antibody accumulates acetylated LDL-derived cholesterol in cultured macrophages

Bis(monoacylglycero)phosphate (BMP), also called lysobisphosphatidic acid, is a phospholipid highly enriched in the internal membranes of multivesicular late endosomes, in which it forms specialized lipid domains. It has been suggested that BMP-rich membranes regulate cholesterol transport. Here, we...

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Published inJournal of lipid research Vol. 48; no. 3; pp. 543 - 52
Main Authors Delton-Vandenbroucke, Isabelle, Bouvier, Jerome, Makino, Asami, Besson, Nelly, Pageaux, Jean-François, Lagarde, Michel, Kobayashi, Toshihide
Format Journal Article
LanguageEnglish
Published American Society for Biochemistry and Molecular Biology 01.03.2007
Subjects
Animals
Antibodies
Cell Line, Tumor
Cell Membrane
Cells, Cultured
Cholesterol Esters
Cholesterol, LDL
Esterification
Filipin
Food and Nutrition
Humans
Life Sciences
Lipid Metabolism
Lipoproteins, LDL
Lysophospholipids
Macrophages
Mice
Microscopy, Fluorescence
Monoglycerides
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Summary:Bis(monoacylglycero)phosphate (BMP), also called lysobisphosphatidic acid, is a phospholipid highly enriched in the internal membranes of multivesicular late endosomes, in which it forms specialized lipid domains. It has been suggested that BMP-rich membranes regulate cholesterol transport. Here, we examine the effects of an anti-BMP antibody on cholesterol metabolism and transport in two macrophage cell lines, RAW 264.7 and THP-1, during loading with acetylated low density lipoprotein (AcLDL). Anti-BMP antibody was internalized and accumulated in both macrophage cell types. Cholesterol staining with filipin and mass measurements indicate that AcLDL-stimulated accumulation of free cholesterol (FC) was enhanced in macrophages that had accumulated the antibody. Unlike the hydrophobic amine U18666A (3-beta-[2-(diethylamino)ethoxy]androst-5-en-17-one), esterification of AcLDL-derived cholesterol by ACAT was not modified after anti-BMP treatment. AcLDL loading led to an increase of FC in the plasma membrane. This increase was further enhanced in anti-BMP-treated macrophages. However, cholesterol efflux to HDL was reduced in antibody-treated cells. These results suggest that the accumulation of anti-BMP antibody alters cholesterol homeostasis in AcLDL-loaded macrophages.
ISSN:0022-2275
DOI:10.1194/jlr.M600266-JLR200