Paper-based electrodes as a tool for detecting ligninolytic enzymatic activities

Lignin is the most important natural source of aromatic compounds. The valorisation of lignin into aromatics requires fractionation steps that can be catalysed by ligninolytic enzymes. However, one of the main limitations of biological lignin fractionation is the low efficiency of biocatalysts; it i...

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Bibliographic Details
Published inBioelectrochemistry (Amsterdam, Netherlands) Vol. 156
Main Authors Fall, Issa, Doumèche, Bastien, Abdellaoui, Sofiene, Rémond, Caroline, Rakotoarivonina, Harivony, Ochs, Marjorie
Format Journal Article
LanguageEnglish
Published Elsevier 2024
Subjects
Biochemistry
Biochemistry, Molecular Biology
Biotechnology
Life Sciences
Depolymerization
Ligninolytic enzymes
Paper -based electrodes
Lignins
Catalase-peroxidase
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Summary:Lignin is the most important natural source of aromatic compounds. The valorisation of lignin into aromatics requires fractionation steps that can be catalysed by ligninolytic enzymes. However, one of the main limitations of biological lignin fractionation is the low efficiency of biocatalysts; it is therefore crucial to enhance or to identify new ligninolytic enzymes. Currently, the screening of ligninolytic activities on lignin polymers represents a technological bottenleck and hinders the characterization and the discovery of efficient ligninolytic biocatalysts. An efficient and fast method for the measurement of such enzymatic activities is therefore required. In this work, we present a new electrochemical tool based on lignin-coated paper electrodes for the detection and the characterization of ligninolytic activity. The suitability of this method is demonstrated using a catalaseperoxidase isolated from Thermobacillus xylanilyticus.
ISSN:1567-5394
DOI:10.1016/j.bioelechem.2023.108609