Materials and Methods Collection and Preparation of Plant Material Aristolochia longa leaves and roots were collected in Mach

• Published in: Journal of Applied Biotechnology Reports Vol. 7; no. 3; pp. 166 - 170
• Main Authors: Attou, Soumia, Meddah, Boumediene, Tir Touil Meddah, Aicha, Mokhtar, Meriem, Sonnet, Pascal
• Format: Journal Article
• Language: English
• Published: Tehran: Baqiyatallah University of Medical Sciences 05.07.2020
• Subjects: Life Sciences; Pharmaceutical sciences; Aristolochia longa; Flavonoids; Algerian West Region; Antioxidant Activity; HPLC
• ISSN: 2322-1186; 2423-5784
• DOI: 10.30491/JABR.2020.109897

Introduction: Aristolochia longa, is widely used as medicinal plant, in the Algerian folk medicine since ancient times and passed through the generations. This study was focuses on the qualitative examination of different phytochemical constituents to determine their antioxidant activities. Materials and Methods: Leaves and roots were extracted by liquid∕liquid extraction technique using the methanol as solvent. The total phenolic and flavonoids content were determined by Folin-Ciocalteu and AlCl 3 methods respectively. The antioxidant activity was evaluated with two distinct methods: DPPH radical scavenging assay and ferric reducing antioxidant power test (FRAP). Then, the high performance liquid chromatography (HPLC) method was performed to analyse leaves and roots n-butanol fractions. Results: Leaves gave significant value of polyphenols (8.580 ± 0.04 mg GAE/g DW). Whereas, n-butanol fraction flavonoids extracted from leaves was observed widely highest in Leaves of Aristolochia longa (4.54±1.94 mg CE/g DW). N-butanol fraction leaves showed a powerful scavenging activity and reducing activity with an IC 50 =0.044±0.001 mg/mL and EC 50 =0.126±0.041 mg/mL respectively. The HPLC analysis of leaves and roots n-butanol fraction revealed different bioactive compounds which they belong to the flavonoids category. Conclusions: The results obtained from this study suggest that A. longa leaves were considered as an important resource of flavonoids, which have an interesting antioxidant power.