SARS-CoV-2 integral membrane proteins shape the serological responses of COVID-19 patients

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has elicited a unique mobilization of the scientific community to develop efficient tools to understand and combat the infection. Like other , SARS-CoV-2 hijacks host cell secretory machinery to produce viral proteins that compose...

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Bibliographic Details
Published iniScience Vol. 24; no. 10
Main Authors Martin, Sophie, Heslan, Christopher, Jégou, Guillaume, Eriksson, Leif A, Le Gallo, Matthieu, Thibault, V., Chevet, Eric, Godey, Florence, Avril, Tony
Format Journal Article
LanguageEnglish
Published Elsevier 29.09.2021
Subjects
Cancer
Life Sciences
Spike
SARS-CoV-2
serology
M
envelope proteins
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Summary:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has elicited a unique mobilization of the scientific community to develop efficient tools to understand and combat the infection. Like other , SARS-CoV-2 hijacks host cell secretory machinery to produce viral proteins that compose the nascent virions; including spike (S), envelope (E), and membrane (M) proteins, the most exposed transmembrane proteins to the host immune system. As antibody response is part of the anti-viral immune arsenal, we investigate the immunogenic potential of S, E, and M using a human cell-based system to mimic membrane insertion and N-glycosylation. Both S and M elicit specific Ig production in patients with SARS-CoV-2. Patients with moderate and severe diseases exhibit elevated Ig responses. Finally, reduced Ig binding was observed with spike G614 compared to D614 variant. Altogether, our assay points toward an unexpected immune response against M and represents a powerful tool to test humoral responses against actively evolving SARS-CoV-2 variants and vaccine effectiveness.
Bibliography:PMCID: PMC8479324
ISSN:2589-0042
DOI:10.1016/j.isci.2021.103185