Poly(adp-ribose) Polymerase-1 Regulates Tracp Gene Promoter Activity During RANKL-Induced Osteoclastogenesis

• Published in: Journal of bone and mineral research Vol. 23; no. 4; pp. 564 - 571
• Main Authors: Beranger, Guillaume E., Momier, David, Rochet, Nathalie, Carle, Georges F., Scimeca, Jean-Claude
• Format: Journal Article
• Language: English
• Published: American Society for Bone and Mineral Research 01.04.2008
• Subjects: Acid Phosphatase; Animals; Biochemistry; Biochemistry, Molecular Biology; Bioengineering; Biomaterials; Cancer; Cell Behavior; Cell Line; Cellular Biology; Electrophoretic Mobility Shift Assay; Gene Expression Regulation, Enzymologic; Genomics; Human health and pathology; Isoenzymes; Life Sciences; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Molecular biology; Osteoclasts; Poly(ADP-ribose) Polymerase Inhibitors; Poly(ADP-ribose) Polymerases; Promoter Regions, Genetic; RANK Ligand; Rhumatology and musculoskeletal system; RNA, Messenger; RNA, Small Interfering; Tartrate-Resistant Acid Phosphatase; Transcription, Genetic

The Tracp gene encodes an acid phosphatase strongly upregulated during osteoclastogenesis on RANKL treatment. Using the mouse osteoclastic model RAW264.7, we studied Tracp gene expression, and we identified PARP-1 as a transcriptional repressor negatively regulated by RANKL during osteoclastogenesis...