Post-embryonic Fish Brain Proliferation Zones Exhibit Neuroepithelial-type Gene Expression Profile

In mammals, neuroepithelial cells play an essential role in embryonic neurogenesis, whereas glial stem cells are the principal source of neurons at post-embryonic stages. By contrast, neuroepithelial-like stem/progenitor (NE) cells have been shown to be present throughout life in teleosts. We used 3...

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Published inStem cells (Dayton, Ohio) Vol. 35; no. 6; pp. 1505 - 1518
Main Authors Dambroise, Emilie, Simion, Matthieu, Bourquard, Thomas, Bouffard, Stéphanie, Rizzi, Barbara, Jaszczyszyn, Yan, Bourge, Mickaël, Affaticati, Pierre, Heuzé, Aurélie, Jouralet, Julia, Edouard, Joanne, Brown, Spencer, Thermes, Claude, Poupon, Anne, Reiter, Eric, Sohm, Frédéric, Bourrat, Franck, Joly, Jean-Stéphane
Format Journal Article
LanguageEnglish
Published Alphamed Press 05.06.2017
Subjects
Cognitive Sciences
Life Sciences
Neurobiology
Neurons and Cognition
Psychology and behavior
Cell cycle
Optic tectum
RNA seq
Microcephaly
DNA repair
Neuroepithelial cell
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Summary:In mammals, neuroepithelial cells play an essential role in embryonic neurogenesis, whereas glial stem cells are the principal source of neurons at post-embryonic stages. By contrast, neuroepithelial-like stem/progenitor (NE) cells have been shown to be present throughout life in teleosts. We used 3-dimensional (3D) reconstructions of cleared transgenic wdr12:GFP medaka brains to demonstrate that this cell type is widespread in juvenile and to identify new regions containing NE cells. We established the gene expression profile of optic tectum (OT) NE cells by cell sorting followed by RNA-seq. Our results demonstrate that most OT NE cells are indeed active stem cells and that some of them exhibit long G2 phases. We identified several novel pathways (e.g., DNA repair pathways) potentially involved in NE cell homeostasis. In situ hybridization studies showed that all NE populations in the post-embryonic medaka brain have a similar molecular signature. Our findings highlight the importance of NE progenitors in medaka and improve our understanding of NE-cell biology. These cells are potentially useful not only for neural stem cell studies, but also for improving the characterization of neurodevelopmental diseases, such as microcephaly. This article is protected by copyright. All rights reserved.
ISSN:1066-6099
1549-4918
DOI:10.1002/stem.2588