Large parallelization of STED nanoscopy using optical lattices

As a scanning microscope, STimulated Emission Depletion (STED) nanoscopy needs parallelization for fast wide-field imaging. Using well-designed optical lattices for depletion together with wide-field excitation and a fast camera for detection, we achieve large parallelization of STED nanoscopy. Wide...

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Bibliographic Details
Published inOptics express Vol. 22; no. 5; pp. 5581 - 5589
Main Authors Yang, Bin, Przybilla, Frédéric, Mestre, Michael, Trebbia, Jean-Baptiste, Lounis, Brahim
Format Journal Article
LanguageEnglish
Published Optical Society of America - OSA Publishing 10.03.2014
Subjects
Optics
Physics
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Summary:As a scanning microscope, STimulated Emission Depletion (STED) nanoscopy needs parallelization for fast wide-field imaging. Using well-designed optical lattices for depletion together with wide-field excitation and a fast camera for detection, we achieve large parallelization of STED nanoscopy. Wide field of view super-resolved images are acquired by scanning over a single unit cell of the optical lattice, which can be as small as 290 nm * 290 nm. Optical Lattice STED (OL-STED) imaging is demonstrated with a resolution down to 70 nm at 12.5 frames per second.
ISSN:1094-4087
1094-4087
DOI:10.1364//OE.22.005581