First Report and Comparative Genomics Analysis of a [bla.sub.OXA-244]-Harboring Escherichia coli Isolate Recovered in the American Continent

The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the [bla.sub.OXA-244] gene identified in Colombia, South America. The 28Eco12 isolate was...

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Published inAntibiotics (Basel) Vol. 8; no. 4
Main Authors Abril, Deisy, Moya, Ingrid Gisell Bustos, Marquez-Ortiz, Ricaurte Alejandro, Montero, Diego Fernando Josa, Rozo, Zayda Lorena Corredor, Molina, Isabel Torres, Gomez, Natasha Vanegas, Escobar-Perez, Javier
Format Journal Article
LanguageEnglish
Published MDPI AG 01.12.2019
Subjects
Base sequence
Beta lactamases
EDTA
Escherichia coli
Genes
Genetic aspects
Genomes
Genomics
Meropenem
Oxalic acid
Physiological aspects
Tetracyclines
Transposons
Colombia
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Summary:The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the [bla.sub.OXA-244] gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other [bla.sub.OXA-244]-harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The [bla.sub.OXA-244] gene was chromosomally encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a [bla.sub.OXA-244]-harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics8040222