Antioxidant Capacity and Acute Testicular Toxicity of Leucaena leucocephala Aqueous Shoot Tips Plus Young Leaves Extracts/Capacidad Antioxidante y Toxicidad Testicular Aguda de Brotes Acuosos con Extracto de Hojas Nuevas de Leucaena leucocephala

This study aimed to determine the antioxidant capacity of the Leucaena leucocephala aqueous shoot tips plus young leaves (LL-spl) extracts among three different fractions (LL-spl 10, 20, and 40 min) and to examine its acute toxicity on male reproductive parameters. The amount of the total phenolics...

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Bibliographic Details
Published inInternational journal of morphology Vol. 34; no. 2; p. 514
Main Authors Burawat, Jaturon, Uabandit, Nongnut, Sripanidkulchai, Bungorn, Nualkaew, Somsak, Iamsaard, Sitthichai
Format Journal Article
LanguageSpanish
Published Universidad de La Frontera, Facultad de Medicina 01.06.2016
Subjects
Analysis
Antioxidants (Nutrients)
Biphenyl (Compound)
Body weight
Methods
Testosterone
Toxicity
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Summary:This study aimed to determine the antioxidant capacity of the Leucaena leucocephala aqueous shoot tips plus young leaves (LL-spl) extracts among three different fractions (LL-spl 10, 20, and 40 min) and to examine its acute toxicity on male reproductive parameters. The amount of the total phenolics in LL-spl extract was determined using a Folin-Ciocalteu reagent method and its antioxidant capacity was analyzed using 1, 1-diphenyl l-2-picrylhydrazyl radical scavenging and ferric reducing antioxidant powder methods. The LL-spl extract fraction with highest antioxidant capacity was used in animal treating. Male rats were divided into three groups (n= 5); control and groups treated with LL-spl 400 and 600 mg/Kg body weight for consecutive 40 days. The results showed that the LL-spl 40 min fraction possessed the highest antioxidant capacity. In addition, the LL-spl 400 and 600 groups showed no differences in weights of body, testis and epididymis, serum testosterone levels, and expression of testicular steroidogenic acute regulatory (StAR) protein. Significantly, LL-spl extract reduced the weight of seminal vesicle, sperm concentration, and seminiferous diameters compared with control. Moreover, LL-spl extract had adverse effect on testicular histology in inducing of seminiferous atrophy and degeneration including dilated blood vessels in interstitial tissue. It was concluded that although LL-spl extract possessing antioxidant capacity, in short term consumptions, it could be toxic to some male reproductive organs especially damaging testicular tissues.
ISSN:0717-9367
0717-9502